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" Bacterial chemosensing: methods and protocols "
/ edited by Michael D. Manson.
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Central Library of the Kurdistan University
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| Material Type
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Latin Book
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| Record Number
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217332
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| Doc. No
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6148
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| Language of Document
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English
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| Title & Author
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Bacterial chemosensing: methods and protocols\ / edited by Michael D. Manson.
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| Publication Statement
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New York, NY: : Springer Science+Business Media, , 2018.
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| Series Statement
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Methods in molecular biology (Clifton, N.J.), 1729.; Springer protocols (Series).
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| Page. NO
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xiii, 389 pages.: : illustrations (chiefly color), tables.
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| ISBN
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978-1-4939-7576-1
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: 978-1-4939-7577-8 (eBook)
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| Bibliographies/Indexes
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Includes bibliographical references and index.
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| Contents
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Part I: Introduction 1. The Diversity of Bacterial Chemosensing Michael D. Manson 2. Transmembrane Signal Transduction in Bacterial Chemosensing Michael D. Manson Part II: Methods for Studying Bacterial Chemotaxis at the Population and Whole-Cell Levels 3. Two Spatial Chemotaxis Assays: The Nutrient-Depleted Chemotaxis Assay and the Agarose-Plug-Bridge Assay Victoria Korolik and Karen M. Ottemann 4. Quantification of Bacterial Chemotaxis Responses at the Mouths of Hydrogel Capillaries Benjamin A. Webb, Timofey Arapov, and Birgit Scharf 5. A Static Microfluidic Device for Investigating the Chemotaxis Response to Stable, Non-Linear Gradients Nitesh Sule, Daniel Penarete-Acosta, Derek L. Englert, and Arul Jayaraman 6. Visualizing Chemoattraction of Planktonic Cells to a Biofilm Sneha Jani 7. Labeling Bacterial Flagella with Fluorescent Dyes Linda Turner and Howard C. Berg Part III: In Vivo Analysis of Receptor Function 8. All-Codon Mutagenesis for Structure-Function Studies of Chemotaxis Signaling Proteins Peter Ames and John S. Parkinson 9. Mutational Analysis of Binding Protein/Chemoreceptor Interactions Andrew L. Seely 10. In Vitro Assay for Measuring Receptor-Kinase Activity in the Bacillus subtilis Chemotaxis Pathway Hanna E. Walukiewicz, George W. Ordal, and Christopher V. Rao 11. FRET Analysis of the Chemotaxis Pathway Response Anja Paulick and Victor Sourjik 12. Monitoring Two-Component Sensor Kinases with a Chemotaxis Signal Readout Run-Zhi Lai and John S. Parkinson 13. Analyzing Protein Domain Interactions in Chemoreceptors by In Vivo PEGylation Kylie J. Watts and Mark S. Johnson 14. Tuning Chemoreceptor Signaling by Positioning Aromatic Residues at the Lipid-Aqueous Interface Rahmi Yusuf, Robert J. Lawrence, Lucy V. Eke, and Roger R. Draheim 15. Analyzing Chemoreceptor Interactions In Vivo with the Tri-Functional Crosslinker TMEA Claudia A. Studdert and Diego A. Massazza Part IV: Cryo-EM Methods for Studying Chemoreceptor Structure 16. Use of Cryo-EM to Study the Structure of Chemoreceptor Arrays In Vivo Wen Yang and Ariane Briegel 17. Visualizing Chemoreceptor Arrays in Bacterial Minicells by Cryo-Electron Tomography and Subtomogram Analysis Zhuan Qin, Bo Hu, and Jun Liu Part V: Monitoring the Intracellular Movement of Chemosensory Proteins 18. Bacterial Chemoreceptor Imaging at High Spatio-Temporal Resolution Using Photoconvertible Fluorescent Proteins Jacopo Solari, Francois Anquez, Katharina Scherer, and Thomas S. Shimizu 19. Imaging of Single Dye-Labeled Chemotaxis Proteins in Live Bacteria Using Electroporation Diana Di Paolo and Richard M. Berry 20. Fluorescence Anisotropy to Detect In Vivo Stimulus-Induced Changes in Chemoreceptor Packing Vered Frank and Ady Vaknin Part VI: High-Throughput Methods for Screening Novel Chemoeffectors 21. Chemotaxis to Atypical Chemoattractants by Soil Bacteria Rebecca E. Parales and Jayna L. Ditty 22. Screening Chemoreceptor-Ligand Interactions by High-Throughput Thermal-Shift Assays Maximilian K.G. Ehrhardt, Suzanne L. Warring, and Monica L. Gerth 23. High-Throughput Screening to Identify Chemoreceptor Ligands Mathilde Fernandez, Alvaro Ortega, Miriam Rico-Jimenez, David Martin-Mora, Abdelali Daddaoua, Miguel. A. Matilla, and Tino Krell 24. Identification of Specific Ligands for Sensory Receptors by Small-Molecule Ligand Arrays and Surface Plasmon Resonance Christopher J. Day and Victoria Korolik Part VII: Creating Chemical Tools for Studying Chemosensory Signal Transduction 25. Fluorescence Measurement of Kinetics of CheY Autophosphorylation with Small Molecule Phosphodonors Ruth E. Silversmith and Robert B. Bourret 26. Synthesis of a Stable Analog of the Phosphorylated Form of CheY: Phosphono-CheY Daniel B. Lookadoo, Matthew S. Beyersdorf, and Christopher J. Halkides Part VIII: Computer Simulation of Chemotaxis 27. Quantitative Modeling of Flagellar Motor-Mediated Adaptation Pushkar P. Lele 28. Molecular Modeling of Chemoreceptor:Ligand Interactions Asuka A. Orr, Arul Jayaraman, and Phanourios Tamamis 29. Phylogenetic and Protein Sequence Analysis of Bacterial Chemoreceptors Davi R. Ortega and Igor B. Zhulin.
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| Abstract
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This volume covers a wide range of up-to-date technologies that have been successfully applied to study the chemosensing behavior of the traditional model species, such as Escherichia coli and Salmonella typhimurium, while being also applicable to a wide spectrum of other species. Beginning with an introduction, the sections of the book explore methods for studying bacterial chemotaxis at the population and whole-cell levels, in vivo analysis of receptor function, cryo-EM methods for studying chemoreceptor structure, as well as intracellular movement of chemosensory proteins, high-throughput methods to screen for novel chemoeffectors, and chemical tools and computer simulations for analyzing chemotaxis. Written for the highly successful Methods in Molecular Biology series, chapters include brief introductions to their topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Bacterial Chemosensing: Methods and Protocols provides an extensive repertoire of approaches that can be extended to understanding chemotaxis, in particular, and chemosensing, in general, in the context of the enormously varied lifestyles adopted in the larger bacterial world.
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| Subject
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Bacterial Physiological Phenomena.
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| Subject
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Chemotaxis.
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| Added Entry
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EditorManson, Michael D.,
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| publication status
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e
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| Keyword
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Bacterial Physiological Phenomena.Chemotaxis.
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