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" Flow cytometry basics for the non-expert "
/ Christine Goetz, Christopher Hammerbeck, Jody Bonnevier.
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Central Library of the Kurdistan University
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| Material Type
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Latin Book
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| Record Number
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217188
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6072
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| Language of Document
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English
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| Main Entry
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Goetz, Christine, , Author
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| Title & Author
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Flow cytometry basics for the non-expert\ / Christine Goetz, Christopher Hammerbeck, Jody Bonnevier.
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| Publication Statement
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Cham, Switzerland: : Springer Nature, , 2018.
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| Series Statement
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Techniques in life science and biomedicine for the non-expert.
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| Page. NO
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xvii, 219 pages.: : illustrations (color), tables.
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| ISBN
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978-3-319-98070-6
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: 978-3-319-98071-3 (eBook)
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| Bibliographies/Indexes
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Includes bibliographical references and index.
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| Contents
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1. Flow Cytometry: Definition, History, and Uses in Biotechnology.- 2. Physics of a Flow Cytometer.- 3. The Language of Flow Cytometry and Experimental Setup.- 4. Fluorochrome Choices for Flow Cytometry.- 5. Compensation.- 6. Primary and Seconardy Antibodies and Their Use in Flow Cytometry.- 7. Experimental Considerations with Data Sets as Examples.- 8. Cell Enrichment.- 9. Surface and Intracellular Staining Protocols for Flow Cytometry.- 10. Troubleshooting.
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| Abstract
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The book divides the topic of flow cytometry into easy to understand sections and covers topics such as the physics behind flow cytometry, flow cytometry lingo, designing flow cytometry experiments and choosing appropriate fluorochromes, compensation, sample preparation and controls and ways to assess cellular function using a variety of flow cytometry assays. Written as a series of chapters whose concepts sequentially build off one another, using the list of materials contained within each section along with the readily reproducible laboratory protocols and tips on troubleshooting that are included, readers should be able to reproduce the data figures presented throughout the book on their way to mastering sound basic flow cytometry techniques.--Amazon.com.
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| Subject
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Flow cytometry.
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Hammerbeck, Christopher, Cllaborator
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Bonnevier, Jody, Cllaborator
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| publication status
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e
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| Keyword
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Flow cytometry.
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